Material Characterization

Dulcoside A

Dulcoside A is one of the associated glycosides found in the stevia plant. It is generally present in preparations of Steviol glycosides at levels lower than stevioside or Rebaudioside A.

  • Chemical formula of Dulcoside A
    13-[2-O-6–deoxy-β-L-mannopyropyranosyl-β–D-glucopyranosyl]oxy] kaur-16-en-18-oic acid β-D-glucopyranosyl ester
  • CAS number
    64432-06-0
  • Chemical formula
    C38H60O17
  • Molecular weight
    788.87

Rebaudioside A

Rebaudioside A is one of the two major Steviol glycosides present in the Stevia leaf and is best known for its clean, sweet taste.Its sweetness intensity is around 200 times that of sugar, but will vary depending on the food or beverage in which it is used. Furthermore, it is very stable under a full spectrum of pH levels, heat and light, all conditions that are found in foods and food preparation.

  • Chemical name of Rebaudioside A
    13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl ester
  • CAS number
    58543-16-1
  • Chemical formula
    C44H70O23
  • Molecular weight
    967.01

Rebaudioside B

Rebaudioside B is one of the associated glycosides found in the stevia plant. It is generally present in preparations of Steviol glycosides at levels lower than stevioside or Rebaudioside A.

  • Chemical name is Rebaudioside B
    (13-[(2-O-β-D-Glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid)
  • CAS number
    58543-17-2
  • Chemical formula
    C38H60O18
  • Molecular weight
    804.88

Rebaudioside C

Rebaudioside C is one of the associated glycosides found in the stevia plant. It is generally present in preparations of Steviol glycosides at levels lower than stevioside or Rebaudioside A.

  • Chemical name of Rebaudioside C
    (13-[(2-O-6–deoxy-β-L-mannopyranosyl-3-O-β–D-glucopyranosyl-β-D-glucopyranosyl)oxy] kaur-16-en-18-oic acid β-D-glucopyranosyl ester)
  • CAS number
    63550-99-2
  • Chemical formula
    C44H70O22
  • Molecular weight
    951.01

Rebaudioside D

Rebaudioside D is one of the associated glycosides found in the stevia plant. It is generally present in preparations of Steviol glycosides at levels lower than stevioside or Rebaudioside A.

  • Chemical name of Rebaudioside D
    (13-[2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl]oxy]kaur-16-en-18-oic acid 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester
  • CAS number
    63279-13-0
  • Chemical formula
    C50H80O28
  • Molecular weight
    1129.15

Rebaudioside E

Rebaudioside E is one of the associated glycosides found in the stevia plant. It is generally present in preparations of Steviol glycosides at levels lower than stevioside or Rebaudioside A.

  • Chemical name of Rebaudioside E
    13-[[2-O-(β-D-Glucopyranosyl)-β-D-glucopyranosyl]oxy]kaur-16-en-18-oic acid 2-O-(β-D-glucopyranosyl)-β-D-glucopyranosyl ester
  • CAS number
    63279-14-1
  • Chemical formula
    C44H70O23
  • Molecular weight
    967.01

Rebaudioside F

Rebaudioside F is one of the associated glycosides found in the stevia plant. It is generally present in preparations of Steviol glycosides at levels lower than stevioside or Rebaudioside A.

  • Chemical name of Rebaudioside F
    13-[(2-O-β-D-xylofurananosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D- glucopyranosyl ester
  • CAS number
    438045-89-7
  • Chemical formula
    C43H68O22
  • Molecular weight
    936.9

Rubusoside

Rubusoside is one of the associated glycosides found in the stevia plant. It is generally present in preparations of Steviol glycosides at levels lower than stevioside or Rebaudioside A.

  • Chemical formula of Rubusoside
    13-[(β-D-Glucopyranosyl)oxy)kaur-16-en-18-oic acid β-D-glucopyranosyl ester
  • CAS number
    64849-39-4
  • Chemical formula
    C32H50O13
  • Molecular weight
    642.73

Steviol (Aglycone)

Steviol (R1 = R2 = H) is the aglycone of the Steviol glycosides.

Steviol Glycosides CAS Number

The Steviol glycosides like the majority of other known molecules have a CAS number. This is a unique, sequential identifier number generated by the Chemical Abstracts Service (CAS).

The CAS numbers for the two main Steviol glycosides:

  • Rebaudioside A: 58543-16-1
  • Stevioside: 57817-89-7

Steviol Glycosides Chemical Formula

This refers to the formula that describes the chemical composition of the Steviol glycoside molecules.

In a standardized format, the atoms are listed followed by a number indicating the total number of those particular atoms in the molecule. For example the chemical formula for stevioside is: C38H60O18, whereas the chemical formula for Rebaudioside A is: C44H70O23, reflecting the fact that Rebaudioside A contains one more glucose molecule (C6H12O6) that is connected by an ester bond (loss of water H2O in the process).

The chemical formula does not contain information about the chemical structure.  

Steviol glycosides chemical name

Steviol glycosides is the name given to a particular class of glycosylated diterpenes that have a Steviol backbone and either combinations of glucose alone or glucose and rhamnose or xylose attached via an ester linkage. The diterpene known as Steviol is the aglycone of Stevia's sweet glycosides.

Steviol Glycosides Molar Mass

This refers to the weight of a mole of an individual Steviol glycoside based on its chemical formula. For example, stevioside has the chemical formula C38H60O18, and therefore knowing the molar masses of each of the atoms listed and the numbers of each of the atoms in the molecule, the mole weight is 804.87, whereas Rebaudioside A, with a chemical formula of C44H70O23, has a mole weight of 967.01.

Steviol Glycosides Structural Formula

Representations of the chemical formulae, but with known bond positions and other spatial information depicted. The structural formula of the Steviol backbone and the types of additional sugar moieties for each of the Steviol glycosides is shown below (from Journal of Applied Glycoscience, Vol. 57 (2010), No. 3, pp.199-209): 

NameR1R2

Stevioside

Glcβ1-

Glcβ1-2 Glcβ1- 

Rebaudioside A   

Glcβ1-Glcβ1-2(Glcβ1-3)Glcβ1-

Rebaudioside B

H-Glcβ1-2(Glcβ1-3)Glcβ1-

Rebaudioside C 

Glcβ1-Rhaα1-2(Glcβ1-3)Glcβ1-

Rebaudioside D

Glcβ1-2Glcβ1-Glcβ1-2(Glcβ1-3)Glcβ1-

Rebaudioside E

Glcβ1-2Glcβ1-Glcβ1-2Glcβ1-
Rebaudioside FGlcβ1-

Xylβ1-2(Glcβ1-3)Glcβ1

Dulcoside A

Glcβ1Rhaα1-2Glcβ1-

Rubusoside 

Glcβ1-Glcβ1-

Steviolbioside

H-Glcβ1-2Glcβ1-

Glc, Rha and Xyl represent, respectively, glucose, rhamnose and xylose sugar moieties.

Steviolbioside

Steviolbioside is one of the associated glycosides found in the stevia plant. It is generally present in preparations of Steviol glycosides at levels lower than stevioside or Rebaudioside A.

  • The chemical name of Steviolbioside
    (13-[(2-O-β-D-Glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid)
  • CAS number
    41093-60-1
  • Chemical formula
    C32H50O13
  • Molecular weight
    642.73  

Stevioside

Stevioside is one of the Steviol glycosides found in the Stevia plant and is of principal interest for its sweetening property.

  • Chemical name of Stevioside
    13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] kaur-16-en-18-oic acid, β-D-glucopyranosyl ester
  • CAS number
    57817-89-7
  • Chemical formula
    C38H60O18
  • Molecular weight
    804.87
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